Mr. Rav Mlait reports

JOURNAL OF ANALYTICAL TOXICOLOGY PUBLISHES PEER-REVIEWED VALIDATION STUDY DEMONSTRATING ACCURACY OF OMEGA LABORATORIES' TEST METHOD FOR DETECTING MARIJUANA IN BREATH SAMPLES USING CANNABIX TECHNOLOGIES' BREATH COLLECTION UNIT

Omega Laboratories Inc. and Cannabix Technologies Inc. have published a peer-reviewed groundbreaking study published by Oxford University Press in the prestigious Journal of Analytical Toxicology titled: "Simultaneous Analysis of Delta9 THC, Delta8 THC, CBD and CBN in Breath Aerosols Collected Using Cannabix Technologies breath collection unit." This marks a major milestone in the advancement of marijuana breath testing technology globally.

The study demonstrates the accuracy and utility of Omega Laboratories' analytical method for detecting cannabinoids in breath samples collected using Cannabix Technologies' breath collection unit and breath cartridges -- further validating the system as the leading solution for identifying recent marijuana use. For employers, law enforcement agencies and safety-sensitive industries, this breath-based testing system offers a reliable, non-invasive solution for detecting recent cannabis consumption. This capability fills a critical gap left by traditional methods, which often detect historical -- rather than recent -- use.

In May of 2024 Omega and Cannabix entered a strategic partnership to accelerate design improvements on the Cannabix breath collection unit (BCU) as well as the laboratory test method. Over the course of 20 months, Cannabix made several key enhancements to the BCU while Omega refined the analytical methodology to test breath for usage of marijuana. These successful efforts were combined into the validation study recently published by the Journal of Analytical Toxicology, which is available on-line.

With medical and recreational marijuana use expanding, identification of recent cannabis use whether via smoking or ingestion has become more critical than ever for workplace drug testing, drug treatment facilities and road safety programs. Detection of recent cannabis use in exhaled breath offers an alternative to the more invasive and intrusive testing methods using blood or urine. Breath testing, long established for alcohol detection, has recently gained attention for drug testing following evidence that multiple substances can be identified in breath after consumption. The detection window in breath correlates with those found in blood and can assist with the identification of recency of use within the last four hours prior to collection.

While alcohol breathalyzers are long-established, cannabis testing remains scientifically complex because THC levels in breath are extremely low. Omega has refined precision testing at these low levels for decades by being experts in utilizing hair samples to detect long-term drug usage. Omega's laboratory validated method combined with the Cannabix breath collection unit has created the most commercially viable, non-invasive system for identifying same-day THC usage.

Publication in the Journal of Analytical Toxicology (JAT) indicates the research on the identification and measurement of toxic substances in biological samples has passed rigorous peer review. It adds to the body of knowledge for the toxicology community. JAT is the official journal of the Society of Forensic Toxicologists (SOFT) and The International Association of Forensic Toxicologists (TIAFT). The method was validated according to ANSI/ASB Standard 036, First Edition 2019 guidelines.

"This new publication reinforces the effectiveness of using breath to detect recent drug use," said Bill Corl, chief executive officer of Omega. "This achievement further demonstrates our expertise in forensic toxicology and our commitment to providing advanced testing solutions."

Omega's method validation builds upon dozens of previously published papers while introducing several new key findings. These include:

1. The ability to simultaneously quantify multiple cannabinoids via breath aerosols including Delta9 tetrahydrocannabinol (Delta9 THC), Delta8 tetrahydrocannabinol (Delta8 THC), cannabidiol (CBD) and cannabinol (CBN).
2. The validation of a robust laboratory method that is quicker and simpler than previous approaches while achieving improved detection sensitivity and precision.
3. The establishment of breath sample stability for up to seven days via the Cannabix breath collection kit.
4. Further confirmation that the concentration versus time trends for THC metabolism assessed from breath correlate with those previously reported from blood.

Due to testing complexity requiring the use of LC-MS/MS, analysis must be performed in a laboratory. Collections performed at the roadside, workplace or in a clinic environment are sent to the laboratory and tested to see if a person had consumed marijuana 15 minutes to four hours prior to an incident. This is a very important tool that can be used in combination with other evidence for roadside and other safety sensitive investigations.

The breath collection process includes an ambient air sample followed by an A and B sample which are collected simultaneously with the use of the Cannabix collection cartridge. Omega screens the A sample using LC-MS/MS then, if presumptively positive, the B sample is also tested using LC-MS/MS. If both samples are positive the ambient air sample is tested to rule out environmental contamination.

Rav Mlait, chief executive officer of Cannabix Technologies, stated: "The peer-reviewed article publication in the prestigious Journal of Analytical Toxicology is a significant milestone for Cannabix and its shareholders. I commend the dedicated efforts of both the Cannabix and Omega teams in advancing our breath-based technology to help improve public safety."

Cannabix is currently working to ramp up manufacturing of the BCU's and breath collection kits. The Cannabix BCU brings several advantages:

1. Omega's validation helps establish the Cannabix BCU as a stable, consistent and portable tool for breath collection. The BCU offers a quick, non-invasive collection method regardless of subject's lung function.
2. The BCU adds several safeguards to the reproducible collection process, including the monitoring of subject's breath pressure and volume, the ability to collect ambient air for specimen validity, and A and B cartridges for a dual testing process.
3. The breath collection kit cartridges used by the BCU are currently the most robust method of sending breath samples for laboratory testing. The sturdy nature of the cartridges combined with shipping stability time helps ensure that samples can be successfully processed once received at Omega Laboratories.

The teams at Omega Laboratories and Cannabix are pleased to partner together so that this innovative, much-needed technology is available for safety-sensitive applications.

About Cannabix Technologies Inc.

Cannabix Technologies is a developer of marijuana and alcohol breath technologies for workplaces, law enforcement and other settings. The marijuana breath test (MBT) targets delta 9 THC (the primary psychoactive ingredient in cannabis) in breath and focuses on recent marijuana use. Cannabix is the developer of the BreathLogix autonomous alcohol screening device for organizations who strive to improve alcohol safety and monitoring.

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