Mr. Rav Mlait reports

CANNABIX'S MSBS MARIJUANA BREATHALYZER TECHNOLOGY DETECTS THC IN BREATH AFTER EDIBLES CONSUMPTION

Cannabix Technologies Inc.'s proprietary laboratory-based marijuana detection equipment (as described below) has detected THC (delta-9-tetrahydrocannabinol) in breath samples after consumption of edibles. Cannabix's mass spectrometer breath sampler (MSBS) technology along with the hand-held breath collection unit (BCU) have been providing consistent results in the detection of THC in breath from smoking. More recently, the technology has been used to detect THC after consumption of edibles containing marijuana. Cannabix has been focused on developing it MSBS as a new, simpler and effective way to test for recent use of marijuana and confirm for THC in breath using gold-standard mass spectrometry (MS).

Highlights and updates:

• MSBS technology has shown the sensitivity in the low picogram range, for THC in breath allowing for detection of THC from smoking and edibles up to four hours after consumption.
• Cannabix's MSBS method for detection of THC in breath has striking differences with conventional, legacy methods.
• Cannabix has initiated discussions with industry-leading forensic laboratory organizations on how to best integrate its novel MSBS hardware into current forensic testing and analysis methods. Scientists at Cannabix are experimenting with a quantification marker (using deuterated delta-9-THC as an internal standard) added to its breath sample cartridge for confirmation and potential quantification of THC in breath.
• Cannabix has recently delivered its BCU to the Friedel Clinic in Montana for testing with subjects.

THC detection after edibles consumption

Cannabis and its extracted active ingredients are commonly mixed into various types of food, including brownies, cookies and candy. After eating an edible containing marijuana, THC is absorbed into the bloodstream through the digestive tract and undergoes a first metabolic pass in the liver before entering general circulation. Metabolism through the liver further reduces the amount of analyte that makes its way through to the breath relative to smoking.

MSBS compared with legacy LCMS methods

The MSBS is a novel method for efficient collection of analytes of low volatility from human breath utilizing liquid secondary adsorption. The concept has successfully demonstrated efficient capturing and releasing of THC. This novel method uses the breath aerosol as a carrier of solid and viscous liquid particle analytes as well as a secondary adsorbent to prevent sample loss from droplet surface contact and deposition. Cannabix scientists observe that the collection efficiency of the MSBS sampling method is thus far providing superior results relative to conventional LCMS forensic methods. Using the Cannabix method the results for a breath sample can be acquired from start to finish within a few minutes without any sample preparation or preconcentration steps. In comparison, legacy methods require up to 15 minutes of solvent extraction, 150 minutes of preconcentration and chromatography, which also takes hours. Furthermore, the acquired sample volume is smaller using Cannabix hardware: five breaths compared with over 10 breaths using legacy methods, which is easier and more convenient (and achievable) for subjects.

MSBS technology and BCU

The company's hand-held breath collection unit and mass spectrometer coupled laboratory MS breath sampler are being used together to provide a new method for drug detection that complements gold-standard mass spectrometry (MS) and significantly simplifies laboratory analysis methods, reduces sample turnaround times (thus minimizing operating costs), while maintaining sensitive, precise results.

About Cannabix Technologies Inc.

Cannabix Technologies is a developer of marijuana breathalyzer technologies for law enforcement and the workplace. Cannabix is working to develop drug-screening devices that will detect THC -- the psychoactive component of marijuana that causes impairment using breath samples. Breath testing for THC would allow employers and law enforcement to identify recent marijuana use that better aligns with impairment. Cannabix devices are in the advanced prototype and preclinical testing stage.

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